Quantitative analysis of human milk oligosaccharides by

capillary electrophoresis.

文章出处：Adv Exp Med Biol. 2000;478:381-2.

PMID: 11065092

UDP-N-acetylglucosamine pyrophosphorylase,

a key enzyme in encysting Giardia,

is allosterically regulated.

Abstract: Giardia synthesizes UDP-GalNAc during cyst wall formation (encystment) via a pathway of inducible enzymes similar to that used to synthesize chitin or peptidoglycan and that includes the UTP-requiring UDP-N-acetylglucosamine pyrophosphorylase. Although it has never been reported as a regulatory enzyme in any system studied to date, kinetic da
……

 High-performance capillary electrophoresis of

 sialylated oligosaccharides of human milk

Abstract: Oligosaccharides in human milk inhibit enteric pathogens in vitro and in vivo. Neutral milk oligosaccharides vary among individuals and over the course of lactation. To study such variation in the acidic milk oligosaccharides, a sensitive, convenient, quantitative method is needed. High-performance capillary electrophoresis of underivatized acidic oligosaccharides with d
……

Rapid method for the determination of amino acids in serum

by capillary electrophoresis.

Abstract: A rapid method for the determination of amino acids in serum is presented. The derivatization of amino acids with 2,4-dinitrofluorobenzene was performed in 0.5 M sodium borate (pH 9.5). The complete separation of derivatives of 16 amino acids and an internal standard (D-norleucine) was achieved within 8 min by capillary zone electrophoresis. The running buffer consisted of 30 mM sodium
……

Abstract: The resolution of structural isomers in mixtures of oligosaccharides is often challenging. Capillary electrophoresis was employed to separate three sets of structural isomers of sialylated oligosaccharides found in human milk and bovine colostrum. Different running buffers were necessary to achieve optimal baseline resolution. To resolve 3'- and 6'-sialyllactoses, 0.2 M aqueous sodium phosphate containing 40% methanol as an organic modifier was used as a running buffer. To resolve 3'- and 6'-sialyllactosamines, 0.4 M aqueous sodium phosphate without
……

《临床医学检验专业技师（医师）系列资格考试习题集》


编者名单

【摘要】 全自动核酸测序分析系统具有遗传信息检测和分析功能，随其应用范围增加，开始进入临床。本文介绍了该仪器的工作原理、结构、功能、操作要点以及临床医学应用。

 【摘要】　目的　建立快速测定人血清中游离氨基酸的方法。
……